Objective：To analyze the pathogenesis and risk factors of cerebral infarction in elderly patientswith anemia Method：380 elderly patients with anemia combined with cerebral infarction were selected as the researchobject， according to the result of hemoglobin on admission， they were divided into the anemia cerebral infarction group（113 cases） and non anemia cerebral infarction nfarction group（ 267 cases）， the history and the relevant examination 13 factorssuch as the single factor analysis， and place the meaningful single factor in the multi-factor Logistic regression analysis.Result：13 risk factors were compared among the anemia group in patients with cerebral infarction and nor anemia group，and we　found that age， gender， NIHSS score， history of coronary heart disease， chronic renal failure， hemorrhagiccerebrovascular disease history， smoking and drinking difference between groups were statistically significant（ P<0.05）.
Compared with the control group， age（ OR 1.03； 95%CI 1.01-1.05）， chronic renal failure（ OR 1.42； 95%CI 1.03-1.92）and hemorrhagic cerebrovascular disease history（ OR 3.42； 95%CI 1.18-9.43） is independent risk factors for cerebralinfarction.Conclusion：Compared with non anemia cerebral infarction group， age， history of chronic renal failure andhemorrhagic cerebrovascular disease were the independent risk factors for cerebral infarction.

2015 Vol. 12　(09):　1-4 [Abstract]（ 523 ） HTML (1 KB)  PDF  (721 KB)  ( 231 )

Objective：To observe clinical effect of canesten clotrimazole vaginal tablet joint born on jun（ vaginalactobacillus bacteria live capsule） treating vulva vagina candida disease during pregnancy（ VVC）. Method： 60 cases ofpregnancy VVC pregnant women were divided into research group and the control group. Control group was given routinetreatment， canesten 1 piece of 0.5 g， deep into the vagina， use before bedtime， single drug used. Research group wasgiven the conventional treatment， canesten 1 piece of 0.5 g， deep into the vagina， used before bedtime， single drug use.4 days to give born on jun 1 grain of deep in the vagina， used before sleep every night， 7 days in a row. The two groupsbefore and after the treatment of vaginal cleanness， vaginal pH value and the cure rate were compared， the therapeuticeffect was observed. Result： The vaginal cleanness Ⅰ-Ⅱ degrees accounted for 90.00%， the cure rate was 90.00% of
research group after the treatment， cleanliness Ⅰ-Ⅱ degrees accounted for 66.67%， the cure rate was 66.67% of controlgroup.The vaginal pH value before and after treatment comparison difference was statistically significant（P<0.05）.Conclusion：For pregnancy VVC， on the basis of antifungal therapy combined with lactobacillus preparation namelycanesten joint born on jun can yet be regarded as a kind of curative effect of treatment， clinical popularization value.

2015 Vol. 12　(09):　7-10 [Abstract]（ 740 ） HTML (1 KB)  PDF  (721 KB)  ( 556 )

Objective：To analyze the clinical curative effect of senile breast cancer with brain metastasis cancerof late course three dimensional conformal radiotherapy combined with paclitaxel chemotherapy.Method： 100 elderlypatients of our hospital in metastatic carcinoma of breast and brain were selected and retrospective analyzed，they wererandomly divided into the control group and the treatment group，50 cases in each group.The control group’s patients weretaken three-dimensional conformal radiation therapy treatment，the treatment group’s patients were taken late coursethree dimensional conformal radiotherapy combined with paclitaxel chemotherapy treatment， the clinical curative effect oftwo groups’ patients were compared and analyzed.Result：The clinical curative effect of treatment group’s patients werebetter than the control group，the difference was statistically significant（P<0.05）.Conclusion： Patients taking threedimensional
conformal radiation therapy combined with paclitaxel chemotherapy treatment， can significantly improve thelife quality of patients，it is worthy of clinical application.

2015 Vol. 12　(09):　14-15 [Abstract]（ 478 ） HTML (1 KB)  PDF  (690 KB)  ( 252 )

Objective：To observe the changes in bulbar conjunctiva microcirculation of acid burned rabbitsduring early burning， to study the regularity and characters of its changing， and changes of hemorheology，then exploreits effect on blood viscosity.Method：The healthy adult rabbits were selected as experimental objects， and were randomlydivided into control group and burn group， duplicate the model of acid burn， when two groups after injury immediately，after injury 30 min，1 h， 2 h， the bulbar conjunctiva microcirculation were observed， blooding when two groups afterinjury immediately， after injury 8， 24， 48 h，the parameters included whole-blood viscosity（ηb）， whole-blood restoreviscosity（ηr）， plasma viscosity（ηp）， erythrocyte sedimentation rate（ESR）， K value of ESR equation（K），hematocrit（HCT）， fibrinogen（FB）， erythrocyte stiffen index（TK）， erythrocyte aggregation index（EAI）， test data
were measurement data，and were analyzed by t test.Result：Total credit value of four phases respectively compared withthe control group， there was significant difference（ P<0.05）. Compared the two groups ηb and ηr， and four phase atthe same shear rate， the value in burn injury group was higher than that in control group（ P<0.05）； compared two groupsof ηp， HCT， ESR， K， FB， EAI， TK， the values of burn group in each phase were higher than those of control group（P<0.05）.Conclusion：After acid burns， bulbar conjunctiva microcirculation changes severely， affecting blood flowvelocity in the blood vessels， leading to changes in blood rheology.

2015 Vol. 12　(09):　20-23 [Abstract]（ 446 ） HTML (1 KB)  PDF  (732 KB)  ( 147 )

Objective： To observe the change pattern of CD4+CD25+ regulatory T cells（ Treg） in asthenospermiainfertile patients and the correlation with sperm quality.Method： The peripheral blood and prostatic fluid specimens werecollected from 60 asthenospermia infertile patients as the observation group and 36 healthy volunteers as the control group.The flow cytometry was used to determine the volume of Treg， intracellular transforming growth factor β1（ TGF-β1）
and interleukin 10（ IL-10） in the peripheral blood and prostatic fluid.The correlation of the above three substances ofsperm viability and motility rate were analysed.Result：Compared with control group，the observation group patients ofTreg， IL-10 and TGF-β1 asthenospermia infertile had significantly lower volume in their peripheral blood and prostatemassage fluid，the differences were statistically significant（P<0.05）.There was positive correlation between Treg
and sperm density， vitality and sex hormone levels separately（r>0.075，P<0.05）.There was no significant differencein the sperm volume of the two groups. The sperm motility rate， sperm velocity and ATP of the observation group wereall obviously lower than those of the control group， the difference was statistically significant（P<0.05）. The Pearsonmethod was used， CD4+CD25+ Treg，TGF-β1 and IL-10 had positive correlation with the index of sperm motility rate，
malformation，velocity，and ATP.Conclusion：The dysimmunity of CD4+CD25+ Treg， which causes excessive damage tothe sperm immunity， is possibly an important immunological mechanism of the asthenospermia infertility.

2015 Vol. 12　(09):　27-29 [Abstract]（ 439 ） HTML (1 KB)  PDF  (712 KB)  ( 140 )

Objective： To analyze the normal young people and people with chronic joint ring implementation ofMRI of temporomandibular joint.Method： Normal youth volunteer 40 cases（ normal group）and the hospital treatmentof temporomandibular joint disorders in patients with chronic mouth play rang 15 cases（ case group）were selected andscanned with the same type of MRI. The same check the articular disc， joint fluid and ankle position of two groups were
compared.Result：19 cases of case group joints， a third of each joint were analyzed through the processus condylaris，center，a third of the three layers held outside oblique sagittal plane. It showed that a double concave and the doubleconcave， in which case group with in a double concave joints 1/3， center， outside double concave 1/3 proportion wererespectively 21.0%（4/19）， 21.0%（4/19）， 10.5%（2/19）； ring group 52.6%（ 10/19） of the articular disc was
scattered flake slightly high signal， after 7 located in the border area， after 2 located in the zone， 1 located in the formerand later bring a border area， there were 9 articular disc high signal characterized by large flake， which all located in8 hypertrophy of articular disc. Two groups of double concave joints plate 3 layer within the silent oblique sagittal 1/3，center， outside 1/3 of the proportion of the differences were statistically significant（ P<0.05）； two groups joint fluid，
number of joints under different scores， according to the comparison of differences were statistically significant afterrank and inspection（P<0.05）. Case group joint plate， within 1/3， center， outside 1/3， oblique sagittal plane weresignificantly before the shift， compared with normal group the difference was statistically significant（ P<0.05）； two groupswithin 1/3， center， outside 1/3， oblique sagittal Drace Angle of comparison， the difference between the two groups hadstatistical significance（P<0.05）.Conclusion： MRI of the implementation of the temporomandibular joint chronic jointcan clearly show the location of the articular disc， how many joint fluid， and is obvious before the shift from the obliquesagittal

2015 Vol. 12　(09):　33-36 [Abstract]（ 617 ） HTML (1 KB)  PDF  (801 KB)  ( 210 )

Objective： To evaluate the efficacy and safety of Clopidogrel Betahistine， Danshen injection inthe treatment of vertebral basilar insufficiency（ Vertebral-basilar artery insufficiency， VBI）. Method： Inclusion andexclusion criteria in strict accordance with VBI patients enrolled 120 cases， according to a list of random numbers into thetreatment group of 60 patients received Betahistine 10-30 mg， 5% dextrose injection or 0.9% sodium chloride injection，a after day 1， Danshen injection， intravenous infusion， a 10-20 mL with 5% glucose injection 100-500 mL diluted afteruse， day 1， and oral Clopidogrelbisulfate tablets， for the first time to give 600 mg， 150 mg/d. The control group receivedbetahistine joint compound Danshen injection. Two consecutive 7-21 d after treatment to assess the extent of the patient’svertigo， and using TCD to detect vertebral-basilar artery blood flow velocity and blood rheology， and follow 30 d， observedthe occurrence of recent TIA or cerebral infarction. Result： The treatment group was 0-2 h from patients and 0-6 heffective accounting effect from patients was 48.3%， 36.7%. The proportion of patients in the control group 0-2 h onsetpatients and 0-6 h onset was 28.3%， 58.3%. Treatment group was efficient， the total efficiency was 65.0% and 93.3%，respectively， in the control group significant efficiency， total efficiency of 41.7% and 75.0 %. There was significantdifference between the twogroups（ P<0.05）. After the triple therapy treatment group left vertebral artery， right vertebralartery， basilar artery blood flow velocity， blood viscosity， plasma viscosity， erythrocyte aggregation index improvedsignificantly better than the control group， a significant difference between the two groups（ P<0.05）. Long-term follow30 d， the treatment group 0 case of TIA or stroke occurred in the control group four cases occurred TIA， 1 case developedinto stroke incidence was 9.2%. There was significant difference between the two groups（ 字2=4.627， P=0.031）. Both
groups during the treatment of liver and kidney function did not change significantly after taking the drug. Treatment group1 patients had neutropenia，gastrointestinal adverse reactions occurred in 2 cases， nausea ， abdominal distension， etc.，shall not affect the continued drug use， the control group had no other adverse reactions. Conclusion： Clopidogrel andBetahistine， Danshen injection can significantly improve the vertebral-basilar artery insufficiency caused by vertigo，adverse reactions rarely occur during treatment.

2015 Vol. 12　(09):　40-43 [Abstract]（ 551 ） HTML (1 KB)  PDF  (734 KB)  ( 311 )

Objective：To probe the value of using real-time three-dimensional ultrasonography for the evaluationof carotid atherosclerosis and the degree of coronary stenosis of patients with coronary heart disease.Method：A total of100 patients were suspected coronary heart disease after had being diagnosed by coronary angiography（CAG）.Theywere divided into two groups.All of these patients undertook carotid artery color doppler ultrasonography（2D） and realtime
three-hree-dimensional ultrasonography（ 3D）within CAG diagnosis，and carotid artery plaques were evaluated.Thedependability between carotid atherosclerosis and coronary atherosclerotic heart disease were studied.Result：There wasstatistical significant difference between coronary heart disease group and control carotid plaques group（P<0.05）.Theplaque detection rate raised significantly as coronary artery lesions involving the count increased.Also statistical difference
was existed between patients between single-branch and multi-branches coronary artery disease（P<0.05）.As coronaryatherosclerosis progressed，carotid plaques also become more and more.And lots of plaques were soft plaques and mixedplaques.Statistical difference existed between 3D and 2D in single-branch or multi-branches coronary artery disease andsuperficial ulcer of soft and mixed plaques detection rate（P<0.05）.There was significant positive linear correlation
between carotid atherosclerosis and coronary atherosclerosis（P<0.05）.The superficial ulcer of plaques detection rateof 3D diagnosis was much better than of 2D diagnosis.Conclusion：Using real-time three-dimensional ultrasonographyfor the evaluation of carotid atherosclerosis， the degree of coronary stenosis patients with coronary heart disease can beevalued.Major adverse cardiovascular events is effectively predicted.

2015 Vol. 12　(09):　46-49 [Abstract]（ 499 ） HTML (1 KB)  PDF  (719 KB)  ( 211 )

Objective：To investigate the dose and efficacy of Penehyclidine hydrochloride in the treatment oforganophosphorus pesticide poisoning.Method：76 patients with organophosphorus pesticide poisoning were randomlydivided into control group and low dose group， both groups were treated with the Penehyclidine hydrochloride andpralidoxime chloride.According to light， middle and severe degree of poisoning， the low dose group was given the firstdose of 0.5-1 mg， 1-2 mg，2-3 mg penehyclidine hydrochloride intramuscular injection， 0.5-1 mg in maintenancetherapy per 8 to 12 hour after penehyclidine hydrochloride etherification.The control group was given the first doseof 1-2 mg，2-4 mg，4-6 mg Penehyclidine hydrochloride intramuscular injection， 1-2 mg in maintenance therapyper 8 to 12 hour after penehyclidine hydrochloride etherification.The medication， time of Penehyclidine hydrochlorideetherification and symptoms disappearance， changes of cholinesterase activity and the occurrence of adverse reactionsof two groups were observed.Result：There were no significant difference of the total efficiency of treatment， time ofPenehyclidine hydrochloride etherification and symptoms disappearance， changes of cholinesterase activity between twogroups（ P>0.05）. Dosage for the first time， total dosage in low dose group were lower than those in the control group（P<0.05）. The incidences of adverse reaction such as dry mouth， restlessness and blurred vision in low dose group werelower than those in control group（ P<0.05）.Conclusion：Low dose of Penehyclidine hydrochloride in the treatment oforganophosphorus pesticide poisoning unchanged the efficacy， reduce adverse reactions， and it is worth to further study.

2015 Vol. 12　(09):　55-58 [Abstract]（ 393 ） HTML (1 KB)  PDF  (719 KB)  ( 202 )

Objective： To analyze the behaviors and habits of TB patients with diabetes mellitus， includingsmoking， drinking， out-door activities and in-door ventilation， then to discuss the relationship between behaviorsand diseases. Method： 284 TB patients with diabetes mellitus in one of the hospitals in Qingdao were selectedasparticipants and were investigated by using cross-section investigation from November 2011 to December 2013. The
trained investigators were responsible for distributing questionnaires to the patients and the questionnaires were involvingpatients’ basic information（ age， gender， height， weight， and blood pressure） and related behavioral habits（ out-dooractivity， ventilation， and stay up in the night） before they started their first treatment.Result： Among the 284 patients，67.3% of them had smoking history. The patients with 30 years smoking history are the most（ 57.6%）； the patients with20-29 years smoking history accounted for 24.6% in total； the patients who smoke for 10-19 years represented 11.0%.In addition， heavy smokers accounted for 74.3% of the TB patients with diabetes mellitus； moderate smokers represented8.9%； 16.8% of them were light smokers. Similarly， 35.9% of the TB patients with diabetes mellitus had drinking history，among them， the drinkers with above 30 years drinking history represented 58.8% of the total patients； patients with 20-29 years drinking represented 27.5%， patients with 10-19 years drinking history represented 9.8%， and patients with lessthan 10 years drinkinghabit represented 3.9%. In the meantime， the number of the patients who did less than 2 hours perday out-door activities was the largest， representing 60.2% of the total patients； patients who did more than 2 hours outdooractivities per day represented 22.2%， and patients who never did out-door activities represented 17.6%. In addition，56.3% patients opened the window for more than 2 hours per day； 34.2% for less than 2 hours per day； 7.7% for less than0.5 hour per day and 1.8% patients did not even have the ventilation habit. Moreover， 59.5% of patients did not stay uplate； 29.2% occasionally stayed up in the night， and only 11.3% of patients often stayed up. Conclusion： The majorityof the TB patients with diabetes mellitus had smoking history as heavy smokers and lacked of out-door activities. Mostpatients did not have ventilation habit.

2015 Vol. 12　(09):　64-66 [Abstract]（ 472 ） HTML (1 KB)  PDF  (732 KB)  ( 223 )

Objective： To study the influence of different body position nursing care of ventilator associatedpneumonia.Method： 90 patients with ICU ventilator ventilation in our hospital were randomly selected from June 2012to June 2014 as the research object， and were randomly divided into control group， observation group A and observationgroup B，30 cases in each group.The control group was treated by routine nursing care， observation group A was treated by
rotational position nursing care， observation group B patients were treated by half decubitus position nursing，the nursingeffect and satisfaction of three groups of patients were compared.Result： The observation group A and observation groupB disease risk were significantly lower than the control group， compared to the results of each group show the statisticallysignificant difference（P<0.05）；the risk of illness in observation group A was significantly lower than in the observation
group B， the difference was statistically significant（P<0.05）， where the rate was 56.7% in the control group，6.7% inobservation group A，26.7% in observation group B.The patients’ ICU length of stay and ventilator time in observationgroup A and observation group B were significantly lower than the control group，and the observation group A was betterthan the observation group B，the differences were statistically significant（P<0.05）.The patients’ care and satisfactionin observation group A and observation group B were higher than the control group ，and observation group A was betterthan observation group B，the differences were statistically significant（P<0.05）.Conclusion： With different bodyposition nursing care，the nursing effect is put in bigger difference， the best care result was rotational position， half lyingposition nursing and rotational position all can to a certain extent， reduce the risk of ventilator associated pneumonia， butalso can effectively improve the effect of the patients’ prognosis and family members’ satisfaction degree for nursing， it hasclinical application value.

2015 Vol. 12　(09):　74-77 [Abstract]（ 598 ） HTML (1 KB)  PDF  (732 KB)  ( 176 )

Objective：To investigate the application effect of evidence-based nursing on advanced malignanttumor with intestinal obstruction.Method： 90 patients in our hospital were selected of the malignant tumor with intestinalobstruction，and they were randomly divided into control group and observation group，45 cases in each group. Thecontrol group was given conventional nursing，and the observation group was received evidence-based nursing.The twogroups of anus exhausted and defecated time，abdominal distension and abdominal pain relief time， hospitalization time，the nursing satisfaction，long-term nursing compliance，long-term quality of life and the treatment of efficient werecompared. Result： After the treatment of different nursing ，the confirmation of nursing observation group in the anusexhausted and defecated time， abdominal distension and abdominal pain relief time，and hospitalization time were shorterthan that of the control group.The nursing satisfaction，long-term nursing compliance， long-term quality of life and thetreatment of efficient were better than that of the control group.Conclusion： Evidence-based nursing has advantages inimproving the patient’s recovery progress， relieving symptoms ， shorten hospitalization time ， improving the quality oflife and the treatment of efficient.Meanwhile，it is helpful to promote communicating with doctors and nurses， nurses andpatients，improve nursing quality and patients compliance.

2015 Vol. 12　(09):　80-83 [Abstract]（ 481 ） HTML (1 KB)  PDF  (731 KB)  ( 339 )

Objective：To explore the identification degree of nursing staff， provide reference for the hospital culture bulding.Method：Quantitative research method was adopted. A self-designed questionnaire was used to investigate 408 nurses. Identification degree to the organizational culture， overall identification degree and consisting of 11 subunit. Result：Identification degree of nursing staff to organizational culture were low， and its different among different gender，age， level of education， seniority， position， title of nursing staff， compared with the nurse without administrative position， nursing administrators identification degree were higher. Conclusion：According to the survey results， the hospital administrators should provide more training opportunities to nursing staff， care for the nurses especially towards clinical nurses， creating a "people-oriented" harmonious hospital organizational culture to increase the nursing staff’s identification degree.

2015 Vol. 12　(09):　86-89 [Abstract]（ 421 ） HTML (1 KB)  PDF  (728 KB)  ( 227 )

Objective：To observe the effect of Zhenqi Fuzheng Decoction on immune function of patients withnasopharyngeal carcinoma radiotherapy.Method：60 cases of nasopharyngeal carcinoma were randomly divided into studygroup and control group， each group had 30 cases. The control group carried out a simple three-dimensional conformalradiotherapy， The study group carried three-dimensional conformal radiotherapy and took Astragalus， Ligustrum lucidum
mainly taking health care prescription decoction at first day， a daily agent， until the end of radiotherapy. Two groups wereobserved before and after treatment in patients with peripheral blood CD3， CD4， CD8， CD4/CD8 and NK lymphocyte datachanges. Result：the study group as compared with before treatment， CD3， CD4， CD4/CD8 and NK cells were decreased，CD8 increased slightly， but the difference was not statistically significant（ P>0.05）； the control group compared withbefore treatment， CD3， CD4， CD4/CD8 and NK cells decreased significantly， CD8 increased significantly， the difference
was statistically significant（ P<0.05）； the study group in comparison to the control group after treatment， CD3， CD4， NKcells decreased significantl（ P<0.05） ，CD8 increased significantly（ P<0.01）.Conclusion：Astragalus， Ligustri Lucididecoction has the function of health care to improve mainly radiotherapy in patients with nasopharyngeal carcinoma cellimmune function， there is of great significance for the maintenance of the follow-up treatment.

2015 Vol. 12　(09):　100-102 [Abstract]（ 467 ） HTML (1 KB)  PDF  (700 KB)  ( 229 )

Objective：To understand the separation condition of infant eye secretions of streptococcuspneumoniae and their antimicrobial susceptibility tests， and provide evidence for clinical rational use of antimicrobialagents. Method： Children eye secretion specimens were cultured and antimicrobial susceptibility test results ofdetection of streptococcus pneumoniae were retrospectively analyzed from January 2008 to January 2014 in our hospitaloutpatient department of ophthalmology. Result： In the 838 secretion specimens of child patients of conjunctivitis， 45pathogens were carried out streptococcus pneumoniae， with a positive rate of 5.4%. The sensitivity rates of Ofloxacin andRifampin were higher， 77.8% and 75.6% respectively， while the drug resistance rate of Erythromycin was the highest，66.7%. Conclusion：Streptococcus pneumoniae is one of the main pathogenic bacteria of ocular infections， accordingto the result of drug sensitivity test， fluoroquinolones eye drops can be used as the drug of choice for the treatment ofpneumococcalinfections of the eye.

2015 Vol. 12　(09):　106-108 [Abstract]（ 471 ） HTML (1 KB)  PDF  (699 KB)  ( 163 )

International experience has showed that the medical association was a good development modeto integrate the medical resources， guarantee the community hospital and general practitioner to act as ＂ healthgatekeeper ＂，change the serious shortage of medical resources in the basic institutions， and release the burden ofpatients for large hospital.Medical Association in certain administrative areas by classification of medical organizationmanagement， the integration of internal resources， the medical service for the masses and utilization of high qualitymedical resources in the area， but there are many barriers and difficulties in the implementation process. Through theanalysis of relevant factors， to explore the way to solve the problem， such as the construction and the medical insurancepayment system of government leading； the GPs’ training； make the visit of the doctor start from the practitionercommunity， and perfect two -way referral system， information system construction and the construction of payment systemof medical insurance，the corresponding suggestion were put forward on the construction and development of ChineseMedical Association.

2015 Vol. 12　(09):　111-114 [Abstract]（ 482 ） HTML (1 KB)  PDF  (718 KB)  ( 257 )

Objective： To explore the method and effect of clinical treatment of ICU patients with severecraniocerebral injury. Method： 82 patients with severe craniocerebral injury in our hospital were randomly selectedfrom January 2010 to January 2013，and were randomly divided into treatment group and control group ， 41 cases in
each group.The control group was given conventional treatment， including nutritional support， hemostatic， by loweringthe intracranial pressure， and improved cerebral circulation， glucocorticoid and correct water electrolyte and acid-basebalance， reasonable use of antibiotics and so on， while the treatment group on the basis of conventional therapy，wasgiven the intracranial pressure monitoring， mechanical ventilation， circulation support and mild hypothermia treatmentthe clinical efficacy of the two groups before and after treatment were comparative analyzed.Result：The treatment grouppatients of good were 23 cases， moderate disability， severe disability and plant survival were respectively 8， 6 and 3cases， 1 cases died， the total effective rate was 75.6%.The control group 41 patients treated with good in 15 cases，moderate disability， severe disability and plant survival were respectively 6， 5 and 8 cases， 7 cases died， the total
effective rate was 51.2%. Compared the total efficiency of two groups， the difference was statistically significant（P<0.05），the effect of the treatment group wassignificantly better than the control group.Conclusion： For patients with severecraniocerebral injury， early diagnosis and treatment， and the treatment of integrated within the ICU， can be achievedremarkable curative effect.

2015 Vol. 12　(09):　117-119 [Abstract]（ 452 ） HTML (1 KB)  PDF  (699 KB)  ( 243 )

Objective：To explore the clinical efficacy of laparoscopic radical trachelectomy and lymphadenectomyin the treatment of early-stage cervical cancer.Method：Patients who had undergone the fertility-sparing treatments inour hospital from 2008 to January 2014 were recruited in this study. The clinical and follow-up records were analyzed.Result：Among the 10 patients，there were 9 cases of squamous cell carcinoma，1 case of and enocarcinoma. 1 case withstage IA1，9 cases with stage IB1（FIGO，2009）. 3 patients underwent laparoscopic lymphadenectomy and radicaltrachelectomy，7 patients underwent laparoscopic lymphadenectomy and vaginal radical trachelectomy，1 case receivedneoadjuvant chemotherapy of 3 courses，1 case received chemotherapy of 2 courses after surgery.All patients underfollow-up with the median follow-up time 21 months，2 pregnancies occurred. Among them，1patient had full-term，1patient was 13 weeks of pregnancy by in vitro fertilization-embryo transfer.1 patient relapsed after the fertility-sparingtreatment.Conclusion：Laparoscopic radical trachelectomy and lymphadenectomy is a safe and effective fertility-sparingtreatment for young women with early-stage cervical cancer.

2015 Vol. 12　(09):　123-125 [Abstract]（ 495 ） HTML (1 KB)  PDF  (689 KB)  ( 256 )

Objective：To investigate the clinical application and effect of amplitude integratedeleetreencephalogram（ aEEG） in the premature infant brain（ HIE）.Method：40 cases of brain injury in preterm infantswhose gestational age was 28-36 weeks（ HIE group）and 18 cases with fetal age of 28-36 weeks premature infants
without brain lesion（ health group），who were admitted to our hospital in March 2013 to March 2014.In 48 hours afterpostpartum， infants in the two groups were monitored by electroencephalography，each successive detection ≥ 2 hours.Amplitude integrated EEG of two groups of preterm infants（ aEEG） were analyzed and compared， the characteristics anddifference were observed.Result：All the aEEG features were found having significantly lower values in brain injuries
group（P<0.05）， such as continuty， amplitude of lower bordercontinuty，sleep-wake cycling and all the features haveclosely association with gestational age.Conclusion：aEEG and brain damage in premature infants has close relationship，therefore，it can timely diagnosis of preterm infants who have brain damage and predict the condition.Because theadvantages of simple operation，less affected by environmental factors，easy to read，aEEG has important clinicalapplication value in the preterm infants with brain injury.

2015 Vol. 12　(09):　131-133 [Abstract]（ 436 ） HTML (1 KB)  PDF  (701 KB)  ( 263 )

Objective：To investigate the clinical application and effect of low dose dopamine combined cedilanidin children pulmonitis complicating congestive heartfailure.Method：60 cases children with pulmonitis complicatingcongestive heart failure were selected，and randomly divided into control group and therapy group，30 cases in eachgroup，all children were taken foundational treatment with inhaling oxygen， preventing asthma and anti-infection.Children
in control group were taken cedilanid，and children in therapy group were taken low dose dopamine combined cedilanid，then parameter of heart function and curative effect were contrasted between two groups.Result：Before treatment，twogroups of left ventricular ejection fraction（LVEF），left pre-ejection period（LPEP），left ventricular ejection time（LVET），and E/A， right pre-ejection period（RPEP）， right ventricular ejection time（ RVET） had no statisticallysignificant difference（P>0.05）.After treatment， LVEF ， LVET， E/A， RVET and clinical effective in the therapy groupwere higher than the control group， LPEP， RPEP in the therapy group were lower than the control group， the differenceswere statistically significant（P<0.05）.Conclusion：Low dose dopamine combined cedilanid can improve heart functionin children pulmonitis complicating congestive heart failure， raise clinical effect and had satisfactory curative effect.

2015 Vol. 12　(09):　136-138 [Abstract]（ 573 ） HTML (1 KB)  PDF  (699 KB)  ( 318 )

Objective： To analyze the clinical curative effect of endometrial polyps resection（ TCRP） combinedNorethisterone on endometrial polyps（ EP），in order to provide the basis for the treatment of EP. Method： 80 cases ofendometrial polyp were divided into observation group and control group with 40 cases in each according to random digittablet. The control group was treated with TCRP therapy， while the observation group was treated with Norethisterone for
3 months after TCRP treatment， each time 30-40 mg， 3 times a day， 22 days/month.All patients were followed up for oneyear to compare the menstrual improvement rate and recurrence rate.Result：Follow up 1 year， the normal menstruationof observation group was 37 cases（92.5%），the recurrence was 1 cases（2.5%）. The menstrual improvement rate andthe recurrence rate of observation were statistically significant difference compared with the control group（P<0.05）.Conclusion：The treatment of TCRP combined Norethisterone on endometrial polyps can significantly improve themenstrual disorders induced by endometrial polyp，and effectively reduce the recurrence of endometrial polyps，which isan ideal treatment method for endometrial polyps.

2015 Vol. 12　(09):　141-143 [Abstract]（ 437 ） HTML (1 KB)  PDF  (700 KB)  ( 228 )

Three-dimensional cell culture in vitro can not only simulate the microenvironment of intercellularsignal transduction between cells and extracellular matrix， but also can reproduce the conditions in cell culture. In recentyears， three-dimensional cell culture technology has increasingly widely used in biomedical research， which becomesa powerful tool for drug toxicity assay， tumor multi-drug resistance， high-throughput screening of anticancer drugs anddrug metabolism. In this paper， we give a brief review of their latest research progress on construction of three-dimensionaltumor spheroid in vitro.

2015 Vol. 12　(09):　147-149 [Abstract]（ 256 ） HTML (1 KB)  PDF  (688 KB)  ( 338 )

In the process of malignant tumor therapy， chemotherapy as a common and main treatment， but it hasside effects. As auxiliary chemotherapy treatment of traditional Chinese medicine increasingly mature， not only can riseto the purpose of the treatment of tumors，but also can relieve symptoms of side effects，improve the quality of life andprolong life.

2015 Vol. 12　(09):　153-156 [Abstract]（ 424 ） HTML (1 KB)  PDF  (719 KB)  ( 509 )