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Abstract Objective：To observe the cancer stem cell like characteristics of CD133+ and CD133- in colon cancer cell line SW480，and to analyze the differences of expressions of Bmi-1 gene in CD133+ and CD133- cells.Method：CD133+ and CD133- cells were sorted by flow cytometry，the abilities of sphere-forming and cell proliferation of CD133+ and CD133- cells in vitro were observed.The expressions of Bmi-1 gene in CD133+ and CD133- cells were detected by Real-time PCR.Result：（1）CD133+ cells accounted for 7.02% in SW480.（2）CD133+ cells were formed spheres within 4 culture days and grow fast in vitro.CD133- cells can not formed spheres，but can adhered and differentiated in medium with serum.In serum-free medium，the abilities of proliferation in CD133+ cells were bigger than those in CD133 cells.（3）The expressions of Bmi-1 mRNA in CD133+ cells were significantly higher than those of CD133- cells.Conclusion：The CD133+ cells account for a little proportion in SW480，but have the higher abilities of proliferation.Bmi-1 mRNA express highly in CD133+ cells，may be related to the carcinogenesis.
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