|
|
|
| The Effect of NF-κB Inhibitor Bay 11-7082 on Proliferation and Apoptosis in Human Pancreatic Cancer Cell Line SW1990/ |
| The First People’s Hospital of Yueyang， Yueyang 414000， China |
|
|
|
Abstract Objective：To study the effect of blocking the NF-κB signaling pathway on proliferation and apoptosis in human pancreatic cancer cell line SW1990 and its possible mechanism.Method：SW1990 cells were treated with different concentrations of NF-κB inhibitor Bay 11-7082（2.5 μmol/L，5 μmol/L 和10 μmol/L）， SW1990 cells morphological changes after treatment were observed under the optical microscope and fluorescence microscopy.CCK-8
assay was used to evaluate the growth influence of Bay 11-7082 on pancreatic cancer SW1990 cells， the flow cytometry was used to determine apoptosis in SW1990 cells.Western blot was used to detect the protein expression of caspase-3 and Bax.Result：A dose-dependent inhibitory effect of Bay 11-7082 on pancreatic cancer SW1990 cells was observed.The result of FCM indicated that treated with Bay 11-7082 exhibited a significant increase of apoptosis in a dose-dependent manner.The expression of caspase-3 and Bax was up-regulated in human pancreatic cancer SW1990 cells after treatment. Conclusion：Bay 11-7082 inhibits the proliferation of SW1990 cells， which is mediated by altering apoptosis-related genes through up-regulation of caspase-3 and Bax.
|
|
|
|
|
|
|
|
|
