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Abstract Objective：To acquaint the status of hepatitis B virus（ HBV） infection in nursing students，and provide data for occupational protection of hepatitis B.Method：Hepatitis B serological markers were measured in nursing and midwifery students of 2012 grade from a college of Guangxi before clinical practice，and questionnaire survey of Hepatitis b vaccination situation was proceeded in those five serological markers negative，statistical results were analyzed.Result：The detection results of 638 nursing students display，the positive rate of HBsAg was 5.96% in total，HBeAg positive rate was 1.10%， HBeAb positive rate was 2.98%；five HBV markers negative in 339 cases， accounted for 53.13% of the total number.The results of a questionnaire survey showed that 79.88% of nursing students thought hepatitis B serological markers detection before practice was necessary， but awareness rate for the hepatitis B vaccine prevention was low.Conclusion：20-22 years old nursing students HBsAg positive rate are lower than the HBsAg of our country carrying rate level， large numbers of people are lack of specific immunity for HBV， and the awareness of hepatitis B vaccination rate is low.The propaganda of knowledge of hepatitis B prevention in nursing students should be increased，high-risk individuals should get vaccinations against hepatitis B or reseed vaccine.
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